ELISA/assay

Human G Protein Coupled Receptor 48 ELISA Kit

MBS723237

96 Strip Wells

675 USD

ELISA Kit

Human G Protein Coupled Receptor 48 ELISA Kit

G Protein Coupled Receptor 48

G6PD; Glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase

GPR48

G6PD; G6PD; G6PD1; G6PD

G6PD_HUMAN

Human

Store all reagents at 2-8 degree C.

Signal Transduction

Intended Uses: This G6PD ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of G6PD in the sample, this G6PD ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus G6PD concentration. The concentration of G6PD in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This G6PD enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for G6PD. Standards or samples are then added to the microtiter plate wells and G6PD if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of G6PD present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for G6PD are added to each well to "sandwich" the G6PD immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain G6PD and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

1203978

AAA92653.1

62,468 Da

Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Disease Pathway 530764!!Glutathione Metabolism Pathway 82973!!Glutathione Metabolism Pathway 198824!!Glutathione Metabolism Pathway 343!!Glycogen Storage Diseases Pathway 980468!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Carbohydrates Pathway 106196

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD