ELISA/assay

Rat Fatty-acid amide hydrolase 1 (FAAH) ELISA Kit

MBS7232294

48-Strip-Wells

440 USD

ELISA Kit

Rat Fatty-acid amide hydrolase 1 (FAAH) ELISA Kit

[Fatty-acid amide hydrolase 1 (FAAH)]

[fatty-acid amide hydrolase 1; Fatty-acid amide hydrolase 1; fatty-acid amide hydrolase 1; oleamide hydrolase 1; anandamide amidohydrolase 1; fatty acid amide hydrolase; Anandamide amidohydrolase 1; Oleamide hydrolase 1]

[FAAH]

[FAAH; FAAH; PSAB; FAAH-1; FAAH1]

FAAH1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of FAAH1. No significant cross-reactivity or interference between FAAH1and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FAAH1and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This FAAH1ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FAAH1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: FAAH1ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FAAH1antibody and an FAAH1-HRP conjugate. The assay sample and buffer are incubated together with FAAH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FAAH1concentration since FAAH1from samples and FAAH1-HRP conjugate compete for the anti-FAAH1antibody binding site. Since the number of sites is limited, as more sites are occupied by FAAH1from the sample, fewer sites are left to bind FAAH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAAH1concentration in each sample is interpolated from this standard curve.

166795287

NP_001432.2

NM_001441.2

63,066 Da

Retrograde Endocannabinoid Signaling Pathway (537443); Retrograde Endocannabinoid Signaling Pathway (539806); Anandamide Degradation Pathway (142420); Anandamide Degradation Pathway (139489)

This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq, Jul 2008]

FAAH: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. Homodimer. Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. Inhibited by O-aryl carbamates and alpha-keto heterocytes. Belongs to the amidase family. Protein type: Membrane protein, integral; Hydrolase; EC 3.5.1.99. Chromosomal Location of Human Ortholog: 1p35-p34. Cellular Component: cytoskeleton; cytoplasm; integral to membrane; endomembrane system. Molecular Function: carbon-nitrogen ligase activity, with glutamine as amido-N-donor; fatty acid amide hydrolase activity; acylglycerol lipase activity. Biological Process: fatty acid catabolic process. Disease: Polysubstance Abuse, Susceptibility To

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415