ELISA/assay

Human C X C chemokine receptor type 6 (CXCR6) ELISA Kit

MBS7232285

48-Strip-Wells

470 USD

ELISA Kit

Human C X C chemokine receptor type 6 (CXCR6) ELISA Kit

C X C chemokine receptor type 6 (CXCR6)

C-X-C chemokine receptor type 6; C-X-C chemokine receptor type 6; C-X-C chemokine receptor type 6; CDw186; CXC-R6; CXCR-6; G protein-coupled receptor; G-protein coupled receptor bonzo; G-protein coupled receptor STRL33; chemokine (C-X-C motif) receptor 6; CDw186; G-protein coupled receptor STRL33; G-protein coupled receptor bonzo; CD_antigen: CD186

CXCR6

CXCR6; CXCR6; BONZO; CD186; STRL33; TYMSTR; BONZO; STRL33; TYMSTR; CXC-R6; CXCR-6

CXCR6_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of CXCR6. No significant cross-reactivity or interference between CXCR6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CXCR6 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CXCR6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CXCR6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: CXCR6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCR6 antibody and an CXCR6-HRP conjugate. The assay sample and buffer are incubated together with CXCR6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCR6 concentration since CXCR6 from samples and CXCR6-HRP conjugate compete for the anti-CXCR6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCR6 from the sample, fewer sites are left to bind CXCR6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCR6 concentration in each sample is interpolated from this standard curve.

5730106

NP_006555.1

NM_006564.1

39,280 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide GPCRs Pathway (198897)

CXCR6: Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 3p21. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; coreceptor activity; C-X-C chemokine receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; viral genome replication; chemotaxis; inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675