ELISA/assay

Human Procollagen Type II ELISA Kit

MBS723227

48-Strip-Wells

470 USD

ELISA Kit

Human Procollagen Type II ELISA Kit

Procollagen Type II

procollagen, type II, alpha 1, isoform CRA_c; Collagen alpha-1(II) chain; collagen alpha-1(II) chain; collagen alpha-1(II) chain; Procollagen II alpha 1; alpha-1 type II collagen; procollagen, type II, alpha 1; collagen type II (Col2A1) gene, enhancer region; collagen, type II, alpha 1; Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin

PC2

Col2a1; Col2a1; CG2A1A; COLLII

CO2A1_RAT

Human

This assay has high sensitivity and excellent specificity for detection of PCOL2. No significant cross-reactivity or interference between PCOL2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCOL2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PCOL2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PCOL2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

. Principle of the assay: PCOL2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PCOL2 antibody and an PCOL2-HRP conjugate. The assay sample and buffer are incubated together with PCOL2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCOL2 concentration since PCOL2 from samples and PCOL2-HRP conjugate compete for the anti-PCOL2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PCOL2 from the sample, fewer sites are left to bind PCOL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCOL2 concentration in each sample is interpolated from this standard curve.

149032179

EDL87091.1

134,570 Da

Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1009926); Collagen Biosynthesis And Modifying Enzymes Pathway (1009925); Collagen Formation Pathway (1009924); ECM-receptor Interaction Pathway (83460); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (219771); Extracellular Matrix Organization Pathway (1009923); Focal Adhesion Pathway (83459)

structural component of the cartilage extracellular matrix [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675