ELISA/assay

Human deoxyribonuclease I ELISA Kit

MBS723225

48-Strip-Wells

470 USD

ELISA Kit

Human deoxyribonuclease I ELISA Kit

deoxyribonuclease I

Human deoxyribonuclease I ELISA Kit; deoxyribonuclease I; deoxyribonuclease I (Human)

DNase I

Dnase-I

Human

This assay has high sensitivity and excellent specificity for detection of DNase I. No significant cross-reactivity or interference between DNase I and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DNase I and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1ng/mL.

Human ELISA Kit

Intended Uses: This DNase I ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DNase I. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay DNase I ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DNase I antibody and an DNase I-HRP conjugate. The assay sample and buffer are incubated together with DNase I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DNase I concentration since DNase I from samples and DNase I-HRP conjugate compete for the anti-DNase I antibody binding site. Since the number of sites is limited, as more sites are occupied by DNase I from the sample, fewer sites are left to bind DNase I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DNase I concentration in each sample is interpolated from this standard curve.

181624

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family. Protein type: Deoxyribonuclease; Motility/polarity/chemotaxis; Secreted; EC 3.1.21.1; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular region; nuclear envelope; nucleus. Molecular Function: protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity. Biological Process: apoptosis; DNA catabolic process, endonucleolytic. Disease: Systemic Lupus Erythematosus

48-Strip-Wells

470 USD

96-Strip-Wells

675