ELISA/assay

Rat Translationally-controlled tumor protein (TPT1) ELISA Kit

MBS7232190

48-Strip-Wells

470 USD

ELISA Kit

Rat Translationally-controlled tumor protein (TPT1) ELISA Kit

Translationally-controlled tumor protein (TPT1)

translationally-controlled tumor protein isoform 1; Translationally-controlled tumor protein; translationally-controlled tumor protein; fortilin; histamine-releasing factor; tumor protein, translationally-controlled 1; Fortilin; Histamine-releasing factor; HRF; p23

TPT1

TPT1; TPT1; HRF; p02; p23; TCTP; TCTP; HRF

TCTP_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of TCTP-1. No significant cross-reactivity or interference between TCTP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TCTP-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This TCTP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TCTP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: TCTP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TCTP-1 antibody and an TCTP-1-HRP conjugate. The assay sample and buffer are incubated together with TCTP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TCTP-1 concentration since TCTP-1 from samples and TCTP-1-HRP conjugate compete for the anti-TCTP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TCTP-1 from the sample, fewer sites are left to bind TCTP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TCTP-1 concentration in each sample is interpolated from this standard curve.

555943903

NP_001273201.1

NM_001286272.1

15,597 Da

PLK1 Signaling Events Pathway (138007)

TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation. Protein type: Microtubule-binding; Calcium-binding. Chromosomal Location of Human Ortholog: 13q14. Cellular Component: multivesicular body; extracellular space; cytoplasm; tubulin complex; nucleus. Molecular Function: protein binding; calcium ion binding. Biological Process: regulation of apoptosis; cellular calcium ion homeostasis; calcium ion transport; response to virus; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675