ELISA/assay

Human Calcium/calmodulin dependent protein kinase type II subunit beta (CAMK2B) ELISA Kit

MBS7231907

48-Strip-Wells

470 USD

ELISA Kit

Human Calcium/calmodulin dependent protein kinase type II subunit beta (CAMK2B) ELISA Kit

Calcium/calmodulin dependent protein kinase type II subunit beta (CAMK2B)

Human Calcium/calmodulin dependent protein kinase type II subunit b (CAMK2B) ELISA Kit

calcium/calmodulin-dependent protein kinase type II subunit beta isoform 8; Calcium/calmodulin-dependent protein kinase type II subunit beta; calcium/calmodulin-dependent protein kinase type II subunit beta; caMK-II subunit beta; CaM-kinase II beta chain; CaM kinase II beta subunit; proline rich calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II beta

CAMK2B

CAMK2B

CAMK2B; CAMK2B; CAM2; CAMK2; CAMKB; CAM2; CAMK2; CAMKB; CaM kinase II subunit beta; CaMK-II subunit beta

KCC2B_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of CAMK-2beta. No significant cross-reactivity or interference between CAMK-2beta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAMK-2beta and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CAMK-2beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CAMK-2beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

. Principle of the assay: CAMK-2beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAMK-2beta antibody and an CAMK-2beta-HRP conjugate. The assay sample and buffer are incubated together with CAMK-2beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMK-2beta concentration since CAMK-2beta from samples and CAMK-2beta-HRP conjugate compete for the anti-CAMK-2beta antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMK-2beta from the sample, fewer sites are left to bind CAMK-2beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK-2beta concentration in each sample is interpolated from this standard curve.

26051218

NP_742081.1

NM_172084.2

57,938 Da

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459)

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

CAMK2B: a widely expressed protein kinase of the calcium/calmodulin-dependent protein kinase (CAMK) group. Functions autonomously after Ca2+/calmodulin-binding and autophosphorylation. Involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban, an endogenous inhibitor of SERCA2. The holoenzyme is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2. Interacts with MPDZ. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle and induced during exercise. Eight isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, CAMK; EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; CAMK2 family. Chromosomal Location of Human Ortholog: 7p14.3-p14.1. Cellular Component: nucleoplasm; sarcoplasmic reticulum membrane; plasma membrane; microtubule organizing center; spindle midzone; cytosol. Molecular Function: calmodulin binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; actin binding; ATP binding. Biological Process: regulation of long-term neuronal synaptic plasticity; regulation of skeletal muscle adaptation; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; regulation of calcium ion transport; signal transduction; protein amino acid phosphorylation; regulation of synaptic transmission, cholinergic; synaptic transmission; inhibitory G-protein coupled receptor phosphorylation; peptidyl-serine phosphorylation; response to cadmium ion; calcium ion transport; regulation of synapse structural plasticity; neuromuscular process controlling balance; G1/S transition of mitotic cell cycle

48-Strip-Wells

470 USD

96-Strip-Wells

675