ELISA/assay

Canine Glutamate carboxypeptidase 2 (FOLH1) ELISA Kit

MBS7231877

48-Strip-Wells

470 USD

ELISA Kit

Canine Glutamate carboxypeptidase 2 (FOLH1) ELISA Kit

Glutamate carboxypeptidase 2 (FOLH1)

glutamate carboxypeptidase 2 isoform 2; Glutamate carboxypeptidase 2; glutamate carboxypeptidase 2; NAALADase I; glutamate carboxylase II; glutamate carboxypeptidase II; membrane glutamate carboxypeptidase; cell growth-inhibiting gene 27 protein; folylpoly-gamma-glutamate carboxypeptidase; prostate specific membrane antigen variant F; pteroylpoly-gamma-glutamate carboxypeptidase; N-acetylated alpha-linked acidic dipeptidase 1; N-acetylated-alpha-linked acidic dipeptidase I; folate hydrolase (prostate-specific membrane antigen) 1; Cell growth-inhibiting gene 27 protein; Folate hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase; FGCP; Glutamate carboxypeptidase II; GCPII; Membrane glutamate carboxypeptidase; mGCP; N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; Prostate-specific membrane antigen; PSM; PSMA; Pteroylpoly-gamma-glutamate carboxypeptidase

FOLH1

FOLH1; FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase; FOLH; NAALAD1; PSM; PSMA; GIG27; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA

FOLH1_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of GCP-2. No significant cross-reactivity or interference between GCP-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GCP-2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GCP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GCP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Priciple of the assay: GCP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCP-2 antibody and an GCP-2-HRP conjugate. The assay sample and buffer are incubated together with GCP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCP-2 concentration since GCP-2 from samples and GCP-2-HRP conjugate compete for the anti-GCP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GCP-2 from the sample, fewer sites are left to bind GCP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCP-2 concentration in each sample is interpolated from this standard curve.

62548858

NP_001014986.1

NM_001014986.1

50,090 Da

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Metabolic Pathways (132956); One Carbon Metabolism Pathway (198756); Vitamin Digestion And Absorption Pathway (199556); Vitamin Digestion And Absorption Pathway (199538)

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]

FOLH1: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.17.21; Protease; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: membrane; integral to plasma membrane; cytoplasm. Molecular Function: dipeptidase activity; peptidase activity; carboxypeptidase activity; metallopeptidase activity; metal ion binding. Biological Process: folic acid and derivative metabolic process; proteolysis

48-Strip-Wells

470 USD

96-Strip-Wells

675