ELISA/assay

Human Collagen Type VII ELISA Kit

MBS723146

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Collagen Type VII ELISA Kit

Collagen Type VII

collagen alpha-1(VII) chain; Collagen alpha-1(VII) chain; collagen alpha-1(VII) chain; collagen alpha-1(VII) chain; LC collagen; long-chain collagen; collagen VII, alpha-1 polypeptide; collagen, type VII, alpha 1; Long-chain collagen; LC collagen

COL7

COL7A1; COL7A1; EBD1; EBR1; EBDCT; LC collagen

CO7A1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of CoL-7. No significant cross-reactivity or interference between CoL-7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CoL-7 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive or Sandwich. Sensitivity: 0.1 ng/mL.

Cell Biology

Intended Uses: This CoL-7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CoL-7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CoL-7 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CoL-7. Standards or samples are then added to the microtiter plate wells and CoL-7 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CoL-7 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CoL-7 are added to each well to “sandwich” the CoL-7 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CoL-7 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CoL-7 concentration in each sample is interpolated from this standard curve.

4502961

NP_000085.1

NM_000094.3

292,267 Da

Anchoring Fibril Formation Pathway (730307); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Extracellular Matrix Organization Pathway (576262); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]

COL7A1: the alpha chain of type VII collagen, an extra-cellular basement membrane protein restricted to the zone beneath stratified squamous epithelia. Type VII collagen fibrils are composed of three identical alpha collagen chains. Forms anchoring fibrils, which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Dystrophic epidermolysis bullosa can result from defects in this protein or an autoimmune response made to type VII collagen. Homotrimer. Interacts with MIA3, facilitating its loading into transport carriers and subsequent secretion. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix; Inhibitor. Chromosomal Location of Human Ortholog: 3p21.1. Cellular Component: extracellular matrix; extracellular space; collagen type VII; endoplasmic reticulum lumen; extracellular region; basement membrane. Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding. Biological Process: extracellular matrix disassembly; collagen catabolic process; epidermis development; extracellular matrix organization and biogenesis; cell adhesion. Disease: Transient Bullous Dermolysis Of The Newborn; Epidermolysis Bullosa Dystrophica, Autosomal Dominant; Nail Disorder, Nonsyndromic Congenital, 8; Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Epidermolysis Bullosa Dystrophica, Pretibial; Epidermolysis Bullosa Pruriginosa; Epidermolysis Bullosa With Congenital Localized Absence Of Skin And Deformity Of Nails

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675