ELISA/assay

Human CASP8 and FADD like apoptosis regulator (CFLAR) ELISA Kit

MBS7230831

48-Strip-Wells

470 USD

ELISA Kit

Human CASP8 and FADD like apoptosis regulator (CFLAR) ELISA Kit

CASP8 and FADD like apoptosis regulator (CFLAR)

CASP8 and FADD-like apoptosis regulator; CASP8 and FADD-like apoptosis regulator; CASP8 and FADD-like apoptosis regulator; usurpin beta; caspase homolog; inhibitor of FLICE; caspase-eight-related protein; MACH-related inducer of toxicity; FADD-like anti-apoptotic molecule; FADD-like antiapoptotic molecule 1; caspase-related inducer of apoptosis; cellular FLICE-like inhibitory protein; caspase-like apoptosis regulatory protein; CASP8 and FADD-like apoptosis regulator; Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; UsurpinCleaved into the following 2 chains:CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12

CFLAR

CFLAR; CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1; CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT

CFLAR_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of CFLAR. No significant cross-reactivity or interference between CFLAR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CFLAR and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CFLAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CFLAR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the assay: CFLAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CFLAR antibody and an CFLAR-HRP conjugate. The assay sample and buffer are incubated together with CFLAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CFLAR concentration since CFLAR from samples and CFLAR-HRP conjugate compete for the anti-CFLAR antibody binding site. Since the number of sites is limited, as more sites are occupied by CFLAR from the sample, fewer sites are left to bind CFLAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFLAR concentration in each sample is interpolated from this standard curve.

12643547

O15519.1

44,112 Da

Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822); Caspase-8 Activation By Cleavage Pathway (105654); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Death Receptor Signalling Pathway (105650); Dimerization Of Procaspase-8 Pathway (105655)

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

CFLAR: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. TNFRSF6 stimulation triggers recruitment to the death- inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle. Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Belongs to the peptidase C14A family. 14 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis. Chromosomal Location of Human Ortholog: 2q33-q34. Cellular Component: cytoplasm; CD95 death-inducing signaling complex; cytosol; lipid raft. Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; death receptor binding; enzyme activator activity; protein complex binding. Biological Process: caspase activation; skeletal muscle development; skeletal muscle atrophy; skeletal myofibril assembly; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; skeletal muscle regeneration; regulation of satellite cell proliferation; proteolysis; activation of NF-kappaB transcription factor; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675