ELISA/assay

Mouse 40S ribosomal protein S3 (RPS3) ELISA Kit

MBS7230811

48-Strip-Wells

470 USD

ELISA Kit

Mouse 40S ribosomal protein S3 (RPS3) ELISA Kit

40S ribosomal protein S3 (RPS3)

40S ribosomal protein S3 isoform 1; 40S ribosomal protein S3; 40S ribosomal protein S3; IMR-90 ribosomal protein S3; ribosomal protein S3

RPS3

RPS3; RPS3; S3; OK/SW-cl.26

RS3_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of RPS3. No significant cross-reactivity or interference between RPS3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPS3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This RPS3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RPS3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Epigenetics and Nuclear Signaling

Principle of the assay: RPS3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPS3 antibody and an RPS3-HRP conjugate. The assay sample and buffer are incubated together with RPS3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPS3 concentration since RPS3 from samples and RPS3-HRP conjugate compete for the anti-RPS3 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPS3 from the sample, fewer sites are left to bind RPS3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPS3 concentration in each sample is interpolated from this standard curve.

378548190

NP_001243731.1

NM_001256802.1

28,487 Da

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); Cap-dependent Translation Initiation Pathway (105967); Cytoplasmic Ribosomal Proteins Pathway (198853); Disease Pathway (530764); Eukaryotic Translation Elongation Pathway (105976); Eukaryotic Translation Initiation Pathway (105966); Eukaryotic Translation Termination Pathway (105978); Formation Of A Pool Of Free 40S Subunits Pathway (105968); Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway (105969); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (105973)

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

RPS3: a component of the 40S ribosomal subunit of eukaryotes. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Overexpressed in colorectal cancer. Protein type: RNA-binding; EC 4.2.99.18; Apoptosis; Ribosomal; Translation. Chromosomal Location of Human Ortholog: 11q13.3-q13.5. Cellular Component: focal adhesion; membrane; mitochondrial inner membrane; cytoplasm; nucleolus; spindle; cytosol; ribonucleoprotein complex; nucleus. Molecular Function: mRNA binding; NF-kappaB binding; protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; structural constituent of ribosome; protein kinase A binding; oxidized purine base lesion DNA N-glycosylase activity; damaged DNA binding; iron-sulfur cluster binding; protein kinase binding. Biological Process: mitosis; SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; transcription, DNA-dependent; apoptosis; viral infectious cycle; translational termination; DNA repair; DNA catabolic process, endonucleolytic; activation of NF-kappaB transcription factor; regulation of translation; cellular protein metabolic process; translational elongation; cell division; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; response to DNA damage stimulus; negative regulation of DNA repair

48-Strip-Wells

470 USD

96-Strip-Wells

675