ELISA/assay

Human Band 3 anion transport protein (SLC4A1) ELISA Kit

MBS7230564

48-Strip-Wells

470 USD

ELISA Kit

Human Band 3 anion transport protein (SLC4A1) ELISA Kit

Band 3 anion transport protein (SLC4A1)

band 3 anion transport protein; Band 3 anion transport protein; band 3 anion transport protein; Diego blood group; Swann blood group; anion exchanger 1; anion exchanger-1; Froese blood group; Wright blood group; Waldner blood group; anion exchange protein 1; erythroid anion exchange protein; erythrocyte membrane protein band 3; solute carrier family 4, anion exchanger, number 1; solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group); solute carrier family 4 (anion exchanger), member 1 (Diego blood group); Anion exchange protein 1; AE 1; Anion exchanger 1; Solute carrier family 4 member 1; CD_antigen: CD233

SLC4A1

SLC4A1; SLC4A1; DI; FR; SW; WD; WR; AE1; WD1; BND3; EPB3; CD233; EMPB3; RTA1A; AE1; DI; EPB3; AE 1; Anion exchanger 1

B3AT_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of SLC4A1. No significant cross-reactivity or interference between SLC4A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC4A1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Neurobiology

Intended Uses: This SLC4A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SLC4A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: SLC4A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SLC4A1 antibody and an SLC4A1-HRP conjugate. The assay sample and buffer are incubated together with SLC4A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC4A1 concentration since SLC4A1 from samples and SLC4A1-HRP conjugate compete for the anti-SLC4A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC4A1 from the sample, fewer sites are left to bind SLC4A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC4A1 concentration in each sample is interpolated from this standard curve.

4507021

NP_000333.1

NM_000342.3

101,792 Da

Bicarbonate Transporters Pathway (119560); Collecting Duct Acid Secretion Pathway (147586); Collecting Duct Acid Secretion Pathway (147560); Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway (645347); Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway (645348); Metabolism Pathway (477135); O2/CO2 Exchange In Erythrocytes Pathway (645346); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway (119559)

The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis. [provided by RefSeq, Jul 2008]

SLC4A1: Band 3 is the major integral glycoprotein of the erythrocyte membrane. Band 3 has two functional domains. Its integral domain mediates a 1:1 exchange of inorganic anions across the membrane, whereas its cytoplasmic domain provides binding sites for cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Defects in SLC4A1 are the cause of elliptocytosis type 4 (EL4). EL4 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SLC4A1 are the cause of spherocytosis type 4 (SPH4); also known as hereditary spherocytosis type 4 (HS4). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, autosomal dominant (AD-dRTA). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with hemolytic anemia (dRTA-HA). A disease characterized by the association of hemolytic anemia with distal renal tubular acidosis, the reduced ability to acidify urine resulting in variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with normal red cell morphology (dRTA-NRC). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Belongs to the anion exchanger (TC 2.A.31) family. Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral. Chromosomal Location of Human Ortholog: 17q21.31. Cellular Component: cortical cytoskeleton; basolateral plasma membrane; integral to plasma membrane; plasma membrane; integral to membrane; Z disc. Molecular Function: protein C-terminus binding; bicarbonate transmembrane transporter activity; protein binding; protein homodimerization activity; protein anchor; chloride transmembrane transporter activity; inorganic anion exchanger activity; anion:anion antiporter activity; anion transmembrane transporter activity; ankyrin binding; actin binding. Biological Process: bicarbonate transport; cellular ion homeostasis; regulation of intracellular pH; ion transport; chloride transport; transmembrane transport; anion transport. Disease: Blood Group--swann System; Blood Group--froese; Renal Tubular Acidosis, Distal, Autosomal Dominant; Blood Group--wright Antigen; Renal Tubular Acidosis, Distal, With Hemolytic Anemia; Spherocytosis, Type 4; Blood Group--diego System; Malaria, Susceptibility To; Blood Group--waldner Type

48-Strip-Wells

470 USD

96-Strip-Wells

675