Rat Aryl Hydrocarbon Receptor Nuclear Translocator 2 ELISA Kit | MyBioSource MBS723042 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Aryl Hydrocarbon Receptor Nuclear Translocator 2 ELISA Kit

catalog :

MBS723042

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS723042

products type :

ELISA Kit

products full name :

Rat Aryl Hydrocarbon Receptor Nuclear Translocator 2 ELISA Kit

products short name :

Aryl Hydrocarbon Receptor Nuclear Translocator 2

other names :

aryl hydrocarbon receptor nuclear translocator 2; Aryl hydrocarbon receptor nuclear translocator 2; aryl hydrocarbon receptor nuclear translocator 2; ARNT protein 2; class E basic helix-loop-helix protein 1; aryl-hydrocarbon receptor nuclear translocator 2; Class E basic helix-loop-helix protein 1; bHLHe1

products gene name :

ARNT2

other gene names :

ARNT2; ARNT2; WEDAS; bHLHe1; BHLHE1; KIAA0307; ARNT protein 2; bHLHe1

uniprot entry name :

ARNT2_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of AHRNT2. No significant cross-reactivity or interference between AHRNT2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AHRNT2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This AHRNT2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AHRNT2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Signal Transduction

products description :

Principle of the assay: AHRNT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AHRNT2 antibody and an AHRNT2-HRP conjugate. The assay sample and buffer are incubated together with AHRNT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AHRNT2 concentration since AHRNT2 from samples and AHRNT2-HRP conjugate compete for the anti-AHRNT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AHRNT2 from the sample, fewer sites are left to bind AHRNT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AHRNT2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

68303555

ncbi acc num :

NP_055677.3

ncbi gb acc num :

NM_014862.3

ncbi mol weight :

77,612 Da

ncbi pathways :

Pathways In Cancer (83105); Renal Cell Carcinoma Pathway (83107); Renal Cell Carcinoma Pathway (519); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting additional roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively. [provided by RefSeq, Dec 2013]

uniprot summary :

ARNT2: Specifically recognizes the xenobiotic response element (XRE). 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Apoptosis; Transcription factor. Chromosomal Location of Human Ortholog: 15q24. Cellular Component: transcription factor complex; cytoplasm; nucleus. Molecular Function: signal transducer activity; DNA binding; aryl hydrocarbon receptor binding; protein heterodimerization activity; transcription factor activity. Biological Process: central nervous system development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; brain development; signal transduction; response to estradiol stimulus; negative regulation of apoptosis. Disease: Webb-dattani Syndrome

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage