ELISA/assay

Human Complement C1q like protein 4 (C1QL4) ELISA Kit

MBS7230001

48-Strip-Wells

440 USD

ELISA Kit

Human Complement C1q like protein 4 (C1QL4) ELISA Kit

[Complement C1q like protein 4 (C1QL4)]

[complement C1q-like protein 4; Complement C1q-like protein 4; complement C1q-like protein 4; C1q/TNF-related protein 11; C1q and tumor necrosis factor-related protein 11; complement component 1, q subcomponent-like 4; C1q and tumor necrosis factor-related protein 11; C1q/TNF-related protein 11]

[C1QL4]

[C1QL4; C1QL4; CTRP11; C1QTNF11; CTRP11; C1q/TNF-related protein 11]

C1QL4_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of C1QL4. No significant cross-reactivity or interference between C1QL4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C1QL4 and all the analogues, therefore, cross reaction may still exist in some cases

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 1.0-25ng/mL. Sensitivity: 1.0-25ng/mL

Cell Biology

Intended Uses: This C1QL4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human C1QL4. Principle of the Assay: C1QL4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C1QL4 antibody and an C1QL4-HRP conjugate. The assay sample and buffer are incubated together with C1QL4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C1QL4 concentration since C1QL4 from samples and C1QL4-HRP conjugate compete for the anti-C1QL4 antibody binding site. Since the number of sites is limited, as more sites are occupied by C1QL4 from the sample, fewer sites are left to bind C1QL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1QL4 concentration in each sample is interpolated from this standard curve.

56606142

NP_001008224.1

NM_001008223.1

24,909 Da

C1QL4: May regulate the number of excitatory synapses that are formed on hippocampus neurons. Has no effect on inhibitory synapses. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12q13.12. Cellular Component: extracellular space; collagen. Molecular Function: identical protein binding. Biological Process: negative regulation of fibroblast proliferation; negative regulation of fat cell differentiation

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415