ELISA/assay

Mouse Heparanase ELISA Kit

MBS722987

48-Strip-Wells

470 USD

ELISA Kit

Mouse Heparanase ELISA Kit

Heparanase

heparanase; Heparanase; heparanase; heparanase-1; endo-glucoronidase; heparanase; Endo-glucoronidase; Heparanase-1; Hpa1

HPA

HPSE; HPSE; HPA; HPA1; HPR1; HSE1; HPSE1; HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Hpa1

HPSE_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of HPA. No significant cross-reactivity or interference between HPA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HPA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This HPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: HPA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HPA. Standards or samples are then added to the microtiter plate wells and HPA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HPA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HPA are added to each well to "sandwich" the HPA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HPA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPA concentration in each sample is interpolated from this standard curve.

5870624

AAD54516.1

42,791 Da

Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355); Glycosaminoglycan Metabolism Pathway (645297); HS-GAG Degradation Pathway (645307); Heparan Sulfate Degradation Pathway (413377); Heparan Sulfate Degradation Pathway (468270); Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway (645304); MPS I - Hurler Syndrome Pathway (685537)

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family. Protein type: Extracellular matrix; Hydrolase; EC 3.2.1.166; Secreted, signal peptide; Secreted; Glycan Metabolism - glycosaminoglycan degradation. Chromosomal Location of Human Ortholog: 4q21.3. Cellular Component: nucleoplasm; lysosomal lumen; intracellular membrane-bound organelle; lysosome; lysosomal membrane; extracellular region; nucleus; lipid raft. Molecular Function: protein dimerization activity; syndecan binding; protein binding; beta-glucuronidase activity; heparanase activity. Biological Process: positive regulation of hair follicle development; positive regulation of protein kinase B signaling cascade; proteoglycan metabolic process; glycosaminoglycan catabolic process; positive regulation of blood coagulation; heparan sulfate proteoglycan catabolic process; glycosaminoglycan metabolic process; cell-matrix adhesion; positive regulation of osteoblast proliferation; carbohydrate metabolic process; pathogenesis; regulation of hair follicle development

48-Strip-Wells

470 USD

96-Strip-Wells

675