catalog number :
MBS722967
products type :
ELISA Kit
products full name :
Rat Second mitochondrial activator of caspase ELISA Kit
products short name :
Second mitochondrial activator of caspase
products gene name :
Smac/DIABLO
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 ng/mL.
products categories :
Cell Biology
products description :
Intended Uses: This Smac/DIABLO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Smac/DIABLO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: Smac/DIABLO ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Smac/DIABLO. Standards or samples are then added to the microtiter plate wells and Smac/DIABLO if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Smac/DIABLO present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Smac/DIABLO are added to each well to "sandwich" the Smac/DIABLO immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Smac/DIABLO and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Smac/DIABLO concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)