ELISA/assay

Human Autophagy related protein 2 homolog A (ATG2A) ELISA Kit

MBS7229592

48-Strip-Wells

470 USD

ELISA Kit

Human Autophagy related protein 2 homolog A (ATG2A) ELISA Kit

Autophagy related protein 2 homolog A (ATG2A)

autophagy-related protein 2 homolog A; Autophagy-related protein 2 homolog A; autophagy-related protein 2 homolog A; ATG2 autophagy related 2 homolog A; autophagy related 2A

ATG2A

ATG2A; ATG2A; KIAA0404

ATG2A_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ATG2A. No significant cross-reactivity or interference between ATG2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG2A and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ATG2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATG2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: ATG2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG2A antibody and an ATG2A-HRP conjugate. The assay sample and buffer are incubated together with ATG2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG2A concentration since ATG2A from samples and ATG2A-HRP conjugate compete for the anti-ATG2A antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG2A from the sample, fewer sites are left to bind ATG2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG2A concentration in each sample is interpolated from this standard curve.

239047271

NP_055919.2

NM_015104.2

13,576 Da

ATG2A: Belongs to the ATG2 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Autophagy. Chromosomal Location of Human Ortholog: 11q13.1. Cellular Component: extrinsic to membrane; pre-autophagosomal structure membrane; lipid particle. Molecular Function: protein binding. Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation

48-Strip-Wells

470 USD

96-Strip-Wells

675