catalog number :
MBS722948
products type :
ELISA Kit
products full name :
Rat Fatty acyl-CoA synthetase ELISA Kit
products short name :
Fatty acyl-CoA synthetase
other names :
fatty acyl CoA synthetase; Fatty acyl CoA synthetase, putative; fatty acyl CoA synthetase; Fatty acyl CoA synthetase, putative
other gene names :
Tb10.70.4200
uniprot entry name :
Q38BS1_TRYB2
specificity :
This assay has high sensitivity and excellent specificity for detection of FACS. No significant cross-reactivity or interference between FACS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FACS and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This FACS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FACS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: FACS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FACS antibody and an FACS-HRP conjugate. The assay sample and buffer are incubated together with FACS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FACS concentration since FACS from samples and FACS-HRP conjugate compete for the anti-FACS antibody binding site. Since the number of sites is limited, as more sites are occupied by FACS from the sample, fewer sites are left to bind FACS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FACS concentration in each sample is interpolated from this standard curve.
ncbi acc num :
XP_822577.1
ncbi gb acc num :
XM_817484.1
ncbi mol weight :
78,984 Da
ncbi pathways :
Fatty Acid Biosynthesis Pathway (84893); Fatty Acid Biosynthesis Pathway (294); Fatty Acid Degradation Pathway (84895); Fatty Acid Degradation Pathway (296); Fatty Acid Metabolism Pathway (869596); Fatty Acid Metabolism Pathway (878045); Metabolic Pathways (132950); Peroxisome Pathway (131223); Peroxisome Pathway (131126); Beta-Oxidation, Acyl-CoA Synthesis Pathway (449285)