Human Melatonin receptor type 1A (MTNR1A) ELISA Kit | MyBioSource MBS7229402 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Melatonin receptor type 1A (MTNR1A) ELISA Kit

catalog :

MBS7229402

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7229402

products type :

ELISA Kit

products full name :

Human Melatonin receptor type 1A (MTNR1A) ELISA Kit

products short name :

Melatonin receptor type 1A (MTNR1A)

other names :

melatonin receptor type 1A; Melatonin receptor type 1A; melatonin receptor type 1A; mel1a receptor; melatonin receptor 1A

products gene name :

MTNR1A

other gene names :

MTNR1A; MTNR1A; MT1; MEL-1A-R; Mel-1A-R; Mel1a receptor

uniprot entry name :

MTR1A_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of MTNR-1A. No significant cross-reactivity or interference between MTNR-1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MTNR-1A and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

products categories :

Neurobiology

products description :

Intended Uses: This MTNR-1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MTNR-1A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay MTNR-1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MTNR-1A antibody and an MTNR-1A-HRP conjugate. The assay sample and buffer are incubated together with MTNR-1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTNR-1A concentration since MTNR-1A from samples and MTNR-1A-HRP conjugate compete for the anti-MTNR-1A antibody binding site. Since the number of sites is limited, as more sites are occupied by MTNR-1A from the sample, fewer sites are left to bind MTNR-1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTNR-1A concentration in each sample is interpolated from this standard curve.

ncbi gi num :

5174593

ncbi acc num :

NP_005949.1

ncbi gb acc num :

NM_005958.3

ncbi mol weight :

39,375 Da

ncbi pathways :

Circadian Entrainment Pathway (698773); Circadian Entrainment Pathway (699872); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Signal Transduction Pathway (477114)

ncbi summary :

This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq, Jul 2008]

uniprot summary :

MTNR1A: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. Protein type: Receptor, GPCR; Motility/polarity/chemotaxis; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral. Chromosomal Location of Human Ortholog: 4q35.1. Cellular Component: integral to plasma membrane; plasma membrane; receptor complex. Molecular Function: protein binding; hormone binding; melatonin receptor activity. Biological Process: circadian rhythm; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of cGMP biosynthetic process; mating behavior

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage