catalog number :
MBS722939
products type :
ELISA Kit
products full name :
Mouse Endotoxin ELISA Kit
products short name :
Endotoxin
other names :
endotoxin; Endotoxin; Endotoxin
other gene names :
cry1Ab
uniprot entry name :
A8WCF4_BACTU
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This EDT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse EDT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Microbiology
products description :
Principle of the Assay: EDT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EDT antibody and an EDT-HRP conjugate. The assay sample and buffer are incubated together with EDT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EDT concentration since EDT from samples and EDT-HRP conjugate compete for the anti-EDT antibody binding site. Since the number of sites is limited, as more sites are occupied by EDT from the sample, fewer sites are left to bind EDT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EDT concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ABW87320.1
ncbi mol weight :
130,752 Da