ELISA/assay

Rat Liver-expressed antimicrobial peptide 2 (LEAP2) ELISA Kit

MBS7229156

48-Strip-Wells

440 USD

ELISA Kit

Rat Liver-expressed antimicrobial peptide 2 (LEAP2) ELISA Kit

[Liver-expressed antimicrobial peptide 2 (LEAP2)]

[liver-expressed antimicrobial peptide 2; Liver-expressed antimicrobial peptide 2; liver-expressed antimicrobial peptide 2; liver expressed antimicrobial peptide 2]

[LEAP2]

[LEAP2; LEAP2; LEAP-2; LEAP-2]

LEAP2_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of LEAP2. No significant cross-reactivity or interference between LEAP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LEAP2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Cardiovascular

Intended Uses: This LEAP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LEAP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: LEAP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LEAP2 antibody and an LEAP2-HRP conjugate. The assay sample and buffer are incubated together with LEAP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LEAP2 concentration since LEAP2 from samples and LEAP2-HRP conjugate compete for the anti-LEAP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by LEAP2 from the sample, fewer sites are left to bind LEAP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LEAP2 concentration in each sample is interpolated from this standard curve.

16418465

NP_443203.1

NM_052971.2

8,814 Da

This gene encodes a cysteine-rich cationic antimicrobial peptide that is expressed predominantly in the liver. The mature peptide has activity against gram-positive bacteria and yeasts. [provided by RefSeq, Sep 2014]

LEAP2: Has an antimicrobial activity. Belongs to the LEAP2 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular region. Biological Process: defense response to bacterium

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415