ELISA/assay

Rat Total Cholesterol ELISA Kit

MBS722885

96 Strip Wells

675 USD

ELISA Kit

Rat Total Cholesterol ELISA Kit

Total Cholesterol

DNAse; DNAse; DNAse

TC

M4LLI9_SALET

Rat

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 0.1 mmoL/L.

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This CH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CH. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY CH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CH antibody and an CH-HRP conjugate. The assay sample and buffer are incubated together with CH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CH concentration since CH from samples and CH-HRP conjugate compete for the anti-CH antibody binding site. Since the number of sites is limited, as more sites are occupied by CH from the sample, fewer sites are left to bind CH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CH concentration in each sample is interpolated from this standard curve.

452120630

YP_007470878.1

NC_020307.1

29,822 Da

96 Strip Wells

675 USD