Human Procollagen C Proteinase Enhancer 1 ELISA Kit | MyBioSource MBS722836 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Procollagen C Proteinase Enhancer 1 ELISA Kit

catalog :

MBS722836

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS722836

products type :

ELISA Kit

products full name :

Human Procollagen C Proteinase Enhancer 1 ELISA Kit

products short name :

Procollagen C Proteinase Enhancer 1

other names :

procollagen C-endopeptidase enhancer 1; Procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer 1; procollagen C-proteinase enhancer 1; procollagen COOH-terminal proteinase enhancer 1; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer; procollagen, type 1, COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer; Procollagen COOH-terminal proteinase enhancer 1; PCPE-1; Procollagen C-proteinase enhancer 1; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer

products gene name :

PCPE1

other gene names :

PCOLCE; PCOLCE; PCPE; PCPE1; PCPE-1; PCPE1; PCPE-1; Procollagen C-proteinase enhancer 1

uniprot entry name :

PCOC1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of PCPE1. No significant cross-reactivity or interference between PCPE1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCPE1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive or Sandwich. Sensitivity: 0.1 ng/mL.

products categories :

Signal Transduction

products description :

Intended Uses: This PCPE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PCPE1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: PCPE1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PCPE1. Standards or samples are then added to the microtiter plate wells and PCPE1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PCPE1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PCPE1 are added to each well to “sandwich” the PCPE1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PCPE1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCPE1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

157653329

ncbi acc num :

NP_002584.2

ncbi gb acc num :

NM_002593.3

ncbi mol weight :

47,972 Da

ncbi pathways :

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]

uniprot summary :

PCOLCE: Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: extracellular matrix; extracellular space. Molecular Function: collagen binding; heparin binding; protein binding; protease activator activity. Biological Process: multicellular organismal development; proteolysis

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage