Human Gelsolin ELISA Kit | MyBioSource MBS7228324 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Gelsolin ELISA Kit

catalog :

MBS7228324

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7228324

products type :

ELISA Kit

products full name :

Human Gelsolin ELISA Kit

products short name :

Gelsolin

other names :

gelsolin; Gelsolin; gelsolin; ADF; brevin; actin-depolymerizing factor; gelsolin; Actin-depolymerizing factor; ADF; Brevin

products gene name :

gel

other gene names :

Gsn; Gsn; ADF

uniprot entry name :

GELS_RAT

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of GS. No significant cross-reactivity or interference between GS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GS and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

other info2 :

Intended Uses: This GS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: GS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GS antibody and an GS-HRP conjugate. The assay sample and buffer are incubated together with GS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GS concentration since GS from samples and GS-HRP conjugate compete for the anti-GS antibody binding site. Since the number of sites is limited, as more sites are occupied by GS from the sample, fewer sites are left to bind GS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GS concentration in each sample is interpolated from this standard curve.

ncbi gi num :

51854227

ncbi acc num :

NP_001004080.1

ncbi gb acc num :

NM_001004080.1

ncbi mol weight :

80,929 Da

ncbi pathways :

Amyloids Pathway (1010489); Apoptosis Pathway (1010630); Apoptotic Cleavage Of Cellular Proteins Pathway (1010658); Apoptotic Execution Phase Pathway (1010657); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1010659); Disease Pathway (1010484); Fc Gamma R-mediated Phagocytosis Pathway (114013); Fc Gamma R-mediated Phagocytosis Pathway (106590); Regulation Of Actin Cytoskeleton Pathway (198525); Regulation Of Actin Cytoskeleton Pathway (83481)

ncbi summary :

plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; may regulate sperm release and turnover of the blood-testis barrier [RGD, Feb 2006]

uniprot summary :

Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation. Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Secreted, signal peptide; Secreted; Actin-binding. Cellular Component: ruffle; extracellular space; protein complex; focal adhesion; lamellipodium; perinuclear region of cytoplasm; extracellular region; cytosol; actin cytoskeleton. Molecular Function: calcium ion binding; actin binding. Biological Process: regulation of cell adhesion; actin filament severing; phosphoinositide-mediated signaling; wound healing; apoptosis; actin nucleation; vesicle-mediated transport; response to ethanol; actin filament polymerization; tissue regeneration; actin polymerization and/or depolymerization; response to folic acid; oligodendrocyte development; barbed-end actin filament capping; aging

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage