ELISA/assay

Mouse Angiogenin ELISA Kit

MBS722813

48-Strip-Wells

470 USD

ELISA Kit

Mouse Angiogenin ELISA Kit

Angiogenin

angiogenin; Angiogenin; Ribonuclease 5; RNase 5

ANG

ANG; RNASE5; RNase 5

ANGI_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This ANG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ANG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: ANG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANG antibody and an ANG-HRP conjugate. The assay sample and buffer are incubated together with ANG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANG concentration since ANG from samples and ANG-HRP conjugate compete for the anti-ANG antibody binding site. Since the number of sites is limited, as more sites are occupied by ANG from the sample, fewer sites are left to bind ANG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG concentration in each sample is interpolated from this standard curve.

178250

AAA51678.1

16,550 Da

ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family. Protein type: Extracellular matrix; EC 3.1.27.-; Cell cycle regulation; RNA-binding; Secreted; Motility/polarity/chemotaxis; Nucleolus; Ribonuclease; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q11.1-q11.2. Cellular Component: angiogenin-PRI complex; extracellular space; growth cone; cell soma; nucleolus; basal lamina; nucleus. Molecular Function: heparin binding; rRNA binding; protein binding; copper ion binding; DNA binding; endonuclease activity; actin binding; peptide binding; ribonuclease activity; receptor binding. Biological Process: cell migration; negative regulation of smooth muscle cell proliferation; diacylglycerol biosynthetic process; activation of protein kinase B; activation of phospholipase A2; response to hormone stimulus; oocyte maturation; rRNA transcription; positive regulation of protein secretion; ovarian follicle development; homeostatic process; phospholipase C activation; actin filament polymerization; negative regulation of translation; response to hypoxia; cell communication; positive regulation of endothelial cell proliferation; angiogenesis; positive regulation of phosphorylation; placenta development. Disease: Amyotrophic Lateral Sclerosis 9

48-Strip-Wells

470 USD

96-Strip-Wells

675