ELISA/assay

Rat C.met Proto-Oncogene Protein ELISA Kit

MBS722809

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat C.met Proto-Oncogene Protein ELISA Kit

C.met Proto-Oncogene Protein

c-Met, partial; Hepatocyte growth factor receptor; hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

C.met

MET; MET; c-Met; HGF receptor; SF receptor

MET_CANFA

Rat

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Intended Uses: This C-met ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat C-met. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This C-met ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat C-met. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the Assay: C-met ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C-met. Standards or samples are then added to the microtiter plate wells and C-met if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C-met present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C-met are added to each well to "sandwich" the C-met immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C-met and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C-met concentration in each sample is interpolated from this standard curve.

25815188

BAC41197.1

154,637 Da

Adherens Junction Pathway (84120); Adherens Junction Pathway (481); Axon Guidance Pathway (84115); Axon Guidance Pathway (476); Axon Guidance Pathway (1016806); Bacterial Invasion Of Epithelial Cells Pathway (149829); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cytokine-cytokine Receptor Interaction Pathway (84101); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (1016805)

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675