ELISA/assay

Rat glycosamionglycan ELISA Kit

MBS722752

48-Strip-Wells

440 USD

ELISA Kit

Rat glycosamionglycan ELISA Kit

[glycosamionglycan]

[Rat glycosamionglycan ELISA Kit; glycosamionglycan; glycosamionglycan (Rat)]

[gag]

[GAG]

Rat

This assay has high sensitivity and excellent specificity for detection of GAG. No significant cross-reactivity or interference between GAG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAG and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 mg/L

Rat ELISA Kit

Intended Uses: This GAG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GAG. Principle of the Assay: GAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GAG antibody and an GAG-HRP conjugate. The assay sample and buffer are incubated together with GAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAG concentration since GAG from samples and GAG-HRP conjugate compete for the anti-GAG antibody binding site. Since the number of sites is limited, as more sites are occupied by GAG from the sample, fewer sites are left to bind GAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAG concentration in each sample is interpolated from this standard curve.

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48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415