ELISA/assay

Mouse BAG family molecular chaperone regulator 2 (BAG2) ELISA Kit

MBS7227510

48-Strip-Wells

470 USD

ELISA Kit

Mouse BAG family molecular chaperone regulator 2 (BAG2) ELISA Kit

BAG family molecular chaperone regulator 2 (BAG2)

BAG family molecular chaperone regulator 2; BAG family molecular chaperone regulator 2; BAG family molecular chaperone regulator 2; bcl-2-associated athanogene 2; BAG-family molecular chaperone regulator-2; dJ417I1.2 (BAG-family molecular chaperone regulator 2); BCL2-associated athanogene 2; Bcl-2-associated athanogene 2

BAG2

BAG2; BAG2; BAG-2; dJ417I1.2; BAG-2

BAG2_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of BAG2. No significant cross-reactivity or interference between BAG2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BAG2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This BAG2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BAG2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: BAG2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BAG2 antibody and an BAG2-HRP conjugate. The assay sample and buffer are incubated together with BAG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAG2 concentration since BAG2 from samples and BAG2-HRP conjugate compete for the anti-BAG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BAG2 from the sample, fewer sites are left to bind BAG2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAG2 concentration in each sample is interpolated from this standard curve.

4757834

NP_004273.1

NM_004282.3

23,772 Da

Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190)

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

BAG2: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Protein type: Apoptosis. Chromosomal Location of Human Ortholog: 6p12.1-p11.2. Molecular Function: identical protein binding; protein binding; chaperone binding. Biological Process: protein folding; protein metabolic process

48-Strip-Wells

470 USD

96-Strip-Wells

675