ELISA/assay

Human Acyloxyacyl hydrolase (AOAH) ELISA Kit

MBS7227379

48-Strip-Wells

470 USD

ELISA Kit

Human Acyloxyacyl hydrolase (AOAH) ELISA Kit

Acyloxyacyl hydrolase (AOAH)

acyloxyacyl hydrolase; Acyloxyacyl hydrolase

AOAH

AOAH

AOAH_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of AOAH. No significant cross-reactivity or interference between AOAH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AOAH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This AOAH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AOAH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: AOAH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AOAH antibody and an AOAH-HRP conjugate. The assay sample and buffer are incubated together with AOAH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AOAH concentration since AOAH from samples and AOAH-HRP conjugate compete for the anti-AOAH antibody binding site. Since the number of sites is limited, as more sites are occupied by AOAH from the sample, fewer sites are left to bind AOAH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOAH concentration in each sample is interpolated from this standard curve.

178069

AAA35506.1

61,647 Da

AOAH: Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Lipase; EC 3.1.1.77. Chromosomal Location of Human Ortholog: 7p14.2. Cellular Component: extracellular region. Molecular Function: lipoprotein lipase activity; acyloxyacyl hydrolase activity; catalytic activity. Biological Process: lipid metabolic process; inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675