ELISA/assay

Rat Cannabinoid Receptor 1, Brain ELISA Kit

MBS722733

48-Strip-Wells

470 USD

ELISA Kit

Rat Cannabinoid Receptor 1, Brain ELISA Kit

Cannabinoid Receptor 1, Brain

cannabinoid receptor 1 isoform a; Cannabinoid receptor 1; cannabinoid receptor 1; central cannabinoid receptor; cannabinoid receptor 1 (brain); CANN6

CNR1

CNR1; CNR1; CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5; CNR; CB-R; CB1

CNR1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of CNR1. No significant cross-reactivity or interference between CNR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNR1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Neurobiology

Intended Uses: This CNR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CNR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CNR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNR1 antibody and an CNR1-HRP conjugate. The assay sample and buffer are incubated together with CNR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNR1 concentration since CNR1 from samples and CNR1-HRP conjugate compete for the anti-CNR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNR1 from the sample, fewer sites are left to bind CNR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNR1 concentration in each sample is interpolated from this standard curve.

237681175

NP_001153731.1

NM_001160259.1

49,060 Da

BDNF Signaling Pathway (712093); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); N-cadherin Signaling Events Pathway (137919); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Rap1 Signaling Pathway (868086)

This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009]

CNR1: Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1. Chromosomal Location of Human Ortholog: 6q14-q15. Cellular Component: growth cone; intracellular membrane-bound organelle; axon; integral to plasma membrane; plasma membrane. Molecular Function: cannabinoid receptor activity; drug binding. Biological Process: response to nicotine; maternal process involved in pregnancy; regulation of synaptic transmission, glutamatergic; negative regulation of fatty acid beta-oxidation; positive regulation of apoptosis; positive regulation of acute inflammatory response to antigenic stimulus; response to morphine; response to lipopolysaccharide; glucose homeostasis; sensory perception of pain; negative regulation of blood pressure; negative regulation of dopamine secretion; response to nutrient; aging; negative regulation of mast cell activation; negative regulation of nitric-oxide synthase activity; axonal fasciculation; negative regulation of action potential; response to cocaine; regulation of synaptic transmission, GABAergic; memory; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to cyclic nucleotide second messenger; response to ethanol; positive regulation of fever; spermatogenesis; regulation of insulin secretion; positive regulation of blood pressure

48-Strip-Wells

470 USD

96-Strip-Wells

675