catalog number :
MBS722671
products type :
ELISA Kit
products full name :
Human MAP kinase phosphatase 1 ELISA Kit
products short name :
MAP kinase phosphatase 1
other names :
MAP kinase phosphatase 1, partial; MAP kinase phosphatase 1; MAP kinase phosphatase 1
products gene name :
MKP 1
other gene names :
MKP1; CHLREDRAFT_44775
uniprot entry name :
A8J529_CHLRE
specificity :
This assay has high sensitivity and excellent specificity for detection of MKP1. No significant cross-reactivity or interference between MKP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MKP1 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1ng/mL.
other info2 :
Intended Uses: This MKP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MKP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
. Principle of the assay: MKP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MKP1 antibody and an MKP1-HRP conjugate. The assay sample and buffer are incubated together with MKP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MKP1 concentration since MKP1 from samples and MKP1-HRP conjugate compete for the anti-MKP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MKP1 from the sample, fewer sites are left to bind MKP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MKP1 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
EDP00867.1
ncbi mol weight :
19,680 Da