ELISA/assay

Guinea pig Muscarinic acetylcholine receptor M3 ELISA Kit

MBS722667

48-Strip-Wells

470 USD

ELISA Kit

Guinea pig Muscarinic acetylcholine receptor M3 ELISA Kit

Muscarinic acetylcholine receptor M3

muscarinic acetylcholine receptor M3; Muscarinic acetylcholine receptor M3; muscarinic acetylcholine receptor M3; m3 muscarinic receptor; acetylcholine receptor, muscarinic 3; cholinergic receptor, muscarinic 3

M-AChr

CHRM3; CHRM3; HM3; EGBRS

ACM3_HUMAN

Guinea Pig

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This CHRM3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig CHRM3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the Assay: CHRM3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CHRM3. Standards or samples are then added to the microtiter plate wells and CHRM3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CHRM3 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CHRM3 are added to each well to "sandwich" the CHRM3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CHRM3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHRM3 concentration in each sample is interpolated from this standard curve.

4502819

NP_000731.1

NM_000740.2

66,128 Da

Acetylcholine Regulates Insulin Secretion Pathway (105924); Amine Ligand-binding Receptors Pathway (106363); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Cholinergic Synapse Pathway (217716); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq, Jul 2008]

mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily. Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q43. Cellular Component: asymmetric synapse; postsynaptic membrane; basolateral plasma membrane; integral to plasma membrane; dendrite; plasma membrane; nerve terminal; cell junction. Molecular Function: protein binding; drug binding; receptor activity; acetylcholine binding; phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity. Biological Process: cell proliferation; nervous system development; G-protein coupled receptor protein signaling pathway; smooth muscle contraction; acetylcholine receptor signaling, muscarinic pathway; regulation of vascular smooth muscle contraction; energy reserve metabolic process; protein modification process; positive regulation of smooth muscle contraction; signal transduction; regulation of insulin secretion; saliva secretion. Disease: Abdominal Muscles, Absence Of, With Urinary Tract Abnormality And Cryptorchidism

48-Strip-Wells

470 USD

96-Strip-Wells

675