Rat 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit | MyBioSource MBS7226302 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit

catalog :

MBS7226302

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7226302

products type :

ELISA Kit

products full name :

Rat 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit

products short name :

14-3-3 protein zeta/delta (YWHAZ)

other names :

14-3-3 protein zeta/delta; 14-3-3 protein zeta/delta; 14-3-3 protein zeta/delta; 14-3-3 zeta; 14-3-3 delta; phospholipase A2; epididymis luminal protein 4; epididymis secretory protein Li 3; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; 14-3-3 protein/cytosolic phospholipase A2; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta; Protein kinase C inhibitor protein 1; KCIP-1

other gene names :

YWHAZ; YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta; KCIP-1

uniprot entry name :

1433Z_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of YWHAZ. No significant cross-reactivity or interference between YWHAZ and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between YWHAZ and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This YWHAZ ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat YWHAZ. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Signal Transduction

products description :

Principle of the assay: YWHAZ ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-YWHAZ antibody and an YWHAZ-HRP conjugate. The assay sample and buffer are incubated together with YWHAZ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAZ concentration since YWHAZ from samples and YWHAZ-HRP conjugate compete for the anti-YWHAZ antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAZ from the sample, fewer sites are left to bind YWHAZ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAZ concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4507953

ncbi acc num :

NP_003397.1

ncbi gb acc num :

NM_003406.3

ncbi mol weight :

19,333 Da

ncbi pathways :

Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Adaptive Immune System Pathway (366160); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Class I PI3K Signaling Events Mediated By Akt Pathway (138020)

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

uniprot summary :

14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization. Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 8q23.1. Cellular Component: extracellular space; focal adhesion; cytoplasmic vesicle membrane; mast cell granule; protein complex; mitochondrion; postsynaptic density; leading edge; cytosol; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus. Molecular Function: identical protein binding; protein domain specific binding; protein binding; protein complex binding; transcription factor binding; protein kinase binding. Biological Process: response to drug; platelet activation; histamine secretion by mast cell; apoptosis; establishment of Golgi localization; gene expression; blood coagulation; signal transduction; protein targeting to mitochondrion; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage