Human plasma kallikrein (KLKB1) ELISA Kit | MyBioSource MBS7226286 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human plasma kallikrein (KLKB1) ELISA Kit

catalog :

MBS7226286

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7226286

products type :

ELISA Kit

products full name :

Human plasma kallikrein (KLKB1) ELISA Kit

products short name :

[plasma kallikrein (KLKB1)]

other names :

[plasma kallikrein; Plasma kallikrein; plasma kallikrein; kininogenin; fletcher factor; kallikrein 3, plasma; plasma prekallikrein; antigen, prostate specific; kallikrein B, plasma 1; Fletcher factor; Kininogenin; Plasma prekallikreinCleaved into the following 2 chains:Plasma kallikrein heavy chain; Plasma kallikrein light chain]

products gene name :

[KLKB1]

other gene names :

[Klkb1; Klkb1; APS; PSA; Kal3; Klk3; Kal-3; Klk3; Pk]

uniprot entry name :

KLKB1_MOUSE

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of KLKB1. No significant cross-reactivity or interference between KLKB1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KLKB1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.

products categories :

Cardiovascular

products description :

Intended Uses: This KLKB1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KLKB1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: KLKB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-KLKB1 antibody and an KLKB1-HRP conjugate. The assay sample and buffer are incubated together with KLKB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLKB1 concentration since KLKB1 from samples and KLKB1-HRP conjugate compete for the anti-KLKB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLKB1 from the sample, fewer sites are left to bind KLKB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLKB1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

236465805

ncbi acc num :

NP_032481.2

ncbi gb acc num :

NM_008455.2

ncbi mol weight :

71,383 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1001029); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (1001028); Extracellular Matrix Organization Pathway (1001015); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1001318); Hemostasis Pathway (1001295); Intrinsic Pathway (1001320)

uniprot summary :

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily. Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.34; Protease. Cellular Component: extracellular space; extracellular region. Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity. Biological Process: fibrinolysis; hemostasis; positive regulation of fibrinolysis; inflammatory response; proteolysis; blood coagulation; plasminogen activation

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage