ELISA/assay

Human Aminoacyl tRNA synthase complex interacting multifunctional protein 1 (SCYE1) ELISA Kit

MBS7226135

48-Strip-Wells

440 USD

ELISA Kit

Human Aminoacyl tRNA synthase complex interacting multifunctional protein 1 (SCYE1) ELISA Kit

[Aminoacyl tRNA synthase complex interacting multifunctional protein 1 (SCYE1)]

[aminoacyl tRNA synthase complex-interacting multifunctional protein 1 isoform b; Aminoacyl tRNA synthase complex-interacting multifunctional protein 1; aminoacyl tRNA synthase complex-interacting multifunctional protein 1; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; multisynthase complex auxiliary component p43; endothelial-monocyte activating polypeptide II; multisynthetase complex auxiliary component p43; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating); aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; Multisynthase complex auxiliary component p43Cleaved into the following chain:Endothelial monocyte-activating polypeptide 2; EMAP-2Alternative name(s):Endothelial monocyte-activating polypeptide II; EMAP-II; Small inducible cytokine subfamily E member 1]

[SCYE1]

[AIMP1; AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII; EMAP2; SCYE1; EMAP-2; EMAP-II]

AIMP1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of SCYE1. No significant cross-reactivity or interference between SCYE1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SCYE1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 250 -5000pg/mL. Sensitivity: 1.0pg/mL

Immunology

Intended Uses: This SCYE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SCYE1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: SCYE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SCYE1 antibody and an SCYE1-HRP conjugate. The assay sample and buffer are incubated together with SCYE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCYE1 concentration since SCYE1 from samples and SCYE1-HRP conjugate compete for the anti-SCYE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SCYE1 from the sample, fewer sites are left to bind SCYE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCYE1 concentration in each sample is interpolated from this standard curve.

215490011

NP_001135888.1

NM_001142416.1

37,039 Da

Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); TRNA Aminoacylation Pathway (105980)

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]

SCYE1 iso2: Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1- mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7. Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: Golgi apparatus; extracellular space; aminoacyl-tRNA synthetase multienzyme complex; transport vesicle; cell surface; membrane; endoplasmic reticulum; cytoplasm; cytosol; nucleus. Molecular Function: methionine-tRNA ligase activity; protein binding; protein homodimerization activity; cytokine activity; tRNA binding. Biological Process: apoptosis; methionyl-tRNA aminoacylation; glucose metabolic process; chemotaxis; signal transduction; tRNA aminoacylation for protein translation; cell-cell signaling; negative regulation of endothelial cell proliferation; response to wounding; gene expression; angiogenesis; inflammatory response; cell adhesion; leukocyte migration. Disease: Leukodystrophy, Hypomyelinating, 3

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415