ELISA/assay

Mouse MHC class I polypeptide-related sequence A ELISA Kit

MBS722574

48-Strip-Wells

470 USD

ELISA Kit

Mouse MHC class I polypeptide-related sequence A ELISA Kit

MHC class I polypeptide-related sequence A

MHC class I polypeptide-related sequence A isoform 2 (MICA*00801); MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence A; HLA class I antigen; stress inducible class I homolog; MHC class I chain-related protein A; MHC class I polypeptide-related sequence A

MICA

MICA; MICA; MIC-A; PERB11.1; PERB11.1; MIC-A

MICA_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of MICA. No significant cross-reactivity or interference between MICA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MICA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0pg/mL.

Immunology

Intended Uses: This MICA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MICA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay MICA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MICA. Standards or samples are then added to the microtiter plate wells and MICA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MICA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MICA are added to each well to "sandwich" the MICA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MICA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MICA concentration in each sample is interpolated from this standard curve.

294660768

NP_001170990.1

NM_001177519.2

31,577 Da

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490)

This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]

MICA: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma. Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the MHC class I family. MIC subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.33. Cellular Component: extracellular space; cell surface; integral to plasma membrane; cytoplasm. Molecular Function: protein binding; beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding. Biological Process: antigen processing and presentation; viral reproduction; response to heat; natural killer cell mediated cytotoxicity; cytolysis; defense response to bacterium; immune response to tumor cell; response to DNA damage stimulus; defense response to virus; gamma-delta T cell activation; T cell mediated cytotoxicity. Disease: Psoriatic Arthritis, Susceptibility To; Psoriasis 1, Susceptibility To

48-Strip-Wells

470 USD

96-Strip-Wells

675