Human 40S ribosomal protein S28 (RPS28) ELISA Kit | MyBioSource MBS7225690 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 40S ribosomal protein S28 (RPS28) ELISA Kit

catalog :

MBS7225690

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7225690

products type :

ELISA Kit

products full name :

Human 40S ribosomal protein S28 (RPS28) ELISA Kit

products short name :

40S ribosomal protein S28 (RPS28)

other names :

40S ribosomal protein S28; 40S ribosomal protein S28; 40S ribosomal protein S28; ribosomal protein S28

products gene name :

RPS28

other gene names :

RPS28; RPS28; S28

uniprot entry name :

RS28_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of RPS28. No significant cross-reactivity or interference between RPS28 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPS28 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This RPS28 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RPS28. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Epigenetics and Nuclear Signaling

products description :

Principle of the assay: RPS28 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPS28 antibody and an RPS28-HRP conjugate. The assay sample and buffer are incubated together with RPS28-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPS28 concentration since RPS28 from samples and RPS28-HRP conjugate compete for the anti-RPS28 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPS28 from the sample, fewer sites are left to bind RPS28-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPS28 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4506715

ncbi acc num :

NP_001022.1

ncbi gb acc num :

NM_001031.4

ncbi mol weight :

7,841 Da

ncbi pathways :

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); Cap-dependent Translation Initiation Pathway (105967); Cytoplasmic Ribosomal Proteins Pathway (198853); Disease Pathway (530764); Eukaryotic Translation Elongation Pathway (105976); Eukaryotic Translation Initiation Pathway (105966); Eukaryotic Translation Termination Pathway (105978); Formation Of A Pool Of Free 40S Subunits Pathway (105968); Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway (105969); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (105973)

ncbi summary :

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S28E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

uniprot summary :

RPS28: Belongs to the ribosomal protein S28e family. Protein type: Translation; RNA-binding; Ribosomal. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: small ribosomal subunit; cytoplasm; cytosol. Molecular Function: structural constituent of ribosome. Biological Process: SRP-dependent cotranslational protein targeting to membrane; ribosomal small subunit biogenesis and assembly; cellular protein metabolic process; translational elongation; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle; rRNA processing. Disease: Diamond-blackfan Anemia 15 With Mandibulofacial Dysostosis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage