Human Caveolin-1 ELISA Kit | MyBioSource MBS722563 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Caveolin-1 ELISA Kit

catalog :

MBS722563

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS722563

products type :

ELISA Kit

products full name :

Human Caveolin-1 ELISA Kit

products short name :

Caveolin-1

products name syn :

Human Caveolin-1 (Cav-1) ELISA Kit; Human Caveolin-1 ELISA Kit; Caveolin-1; Caveolin-1 (Human)

other names :

caveolin-1 isoform 2; Caveolin-1; caveolin-1; caveolin, caveolae protein 1; caveolin, caveolae protein, 22 kDa; caveolin 1, caveolae protein

products gene name :

Cav-1

other gene names :

Cav1; Cav1; Cav; Cav-1; Cav

uniprot entry name :

CAV1_MOUSE

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of CAV-1. No significant cross-reactivity or interference between CAV-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAV-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

products categories :

Human ELISA Kit

products description :

Intended Uses: This CAV-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CAV-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CAV-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CAV-1. Standards or samples are then added to the microtiter plate wells and CAV-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CAV-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CAV-1 are added to each well to "sandwich" the CAV-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CAV-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAV-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

340139110

ncbi acc num :

NP_001229993.1

ncbi gb acc num :

NM_001243064.1

uniprot acc num :

P49817

ncbi mol weight :

20,539 Da

ncbi pathways :

Androgen Receptor Signaling Pathway (198319); Bacterial Invasion Of Epithelial Cells Pathway (149817); Bacterial Invasion Of Epithelial Cells Pathway (148661); Basigin Interactions Pathway (819736); Cell Surface Interactions At The Vascular Wall Pathway (819734); EGFR1 Signaling Pathway (198278); Endocytosis Pathway (102286); Endocytosis Pathway (102181); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264)

uniprot summary :

Function: Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Ref.7. Subunit structure: Homooligomer . By similarity. Interacts (via the N-terminus) with DPP4; the interaction is direct . By similarity. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Interacts with BMX, BTK, CTNNB1, CDH1, GLIPR2, JUP, NOSTRIN, SNAP25 and STX1A . By similarity. Interacts with PACSIN2. Ref.11. Subcellular location: Golgi apparatus membrane; Peripheral membrane protein . By similarity. Cell membrane; Peripheral membrane protein . By similarity. Membrane caveola; Peripheral membrane protein. Membrane raft . By similarity. Note: Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae . By similarity. Ref.11. Post-translational modification: The N-terminus of both isoforms are blocked.Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress. Ref.8 Ref.9. Sequence similarities: Belongs to the caveolin family.

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage