catalog number :
MBS722559
products type :
ELISA Kit
products full name :
Mouse Heat Shock Protein 70 ELISA Kit
products short name :
Heat Shock Protein 70
other names :
HSP70; HSP70; HSP70
products gene name :
HSP70
uniprot entry name :
Q40058_HORVU
specificity :
This assay has high sensitivity and excellent specificity for detection of HSP70. No significant cross-reactivity or interference between HSP70 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP70 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.
products categories :
Signal Transduction
products description :
Principle of the assay: HSP70 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HSP70. Standards or samples are then added to the microtiter plate wells and HSP70 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HSP70 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HSP70 are added to each well to “sandwich” the HSP70 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HSP70 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP70 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAA62325.1
ncbi mol weight :
67,016 Da
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)