ELISA/assay

Mouse Corin (Atrial natriuretic peptide-converting enzyme) ELISA Kit

MBS722545

48-Strip-Wells

470 USD

ELISA Kit

Mouse Corin (Atrial natriuretic peptide-converting enzyme) ELISA Kit

Corin (Atrial natriuretic peptide-converting enzyme)

corin; Atrial natriuretic peptide-converting enzyme; atrial natriuretic peptide-converting enzyme; pro-ANP-convertase; pro-ANP-converting enzyme; transmembrane protease serine 10; heart-specific serine proteinase ATC2; corin, serine peptidase; Corin; Heart-specific serine proteinase ATC2; Pro-ANP-converting enzyme; Transmembrane protease serine 10Cleaved into the following 5 chains:Atrial natriuretic peptide-converting enzyme, N-terminal propeptide; Atrial natriuretic peptide-converting enzyme, activated protease fragment; Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment; Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment; Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment

Corin

CORIN; CORIN; CRN; ATC2; Lrp4; PEE5; TMPRSS10; CRN; TMPRSS10

CORIN_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of CRA-ANPCE. No significant cross-reactivity or interference between CRA-ANPCE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRA-ANPCE and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CRA-ANPCE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CRA-ANPCE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: CRA-ANPCE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRA-ANPCE antibody and an CRA-ANPCE-HRP conjugate. The assay sample and buffer are incubated together with CRA-ANPCE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRA-ANPCE concentration since CRA-ANPCE from samples and CRA-ANPCE-HRP conjugate compete for the anti-CRA-ANPCE antibody binding site. Since the number of sites is limited, as more sites are occupied by CRA-ANPCE from the sample, fewer sites are left to bind CRA-ANPCE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRA-ANPCE concentration in each sample is interpolated from this standard curve.

4884872

AAD31850.1

113,297 Da

Myometrial Relaxation And Contraction Pathways (198759)

This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

CORIN: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Defects in CORIN are the cause of pre-eclampsia/eclampsia 5 (PEE5); also known as gestational proteinuric hypertension. Preeclampsia is a pregnancy-associated disease with maternal symptoms but placental origin. Unlike most other human disorders, it impacts 2 individuals, the mother and her child, both of whom can be severely affected. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; EC 3.4.21.-; Protease; Membrane protein, integral. Chromosomal Location of Human Ortholog: 4p12. Cellular Component: cell surface; integral to plasma membrane; plasma membrane; extracellular region; integral to membrane. Molecular Function: serine-type endopeptidase activity. Biological Process: regulation of blood pressure; peptide hormone processing; female pregnancy; proteolysis; regulation of systemic arterial blood pressure by atrial natriuretic peptide. Disease: Preeclampsia/eclampsia 5

48-Strip-Wells

470 USD

96-Strip-Wells

675