ELISA/assay

Mouse Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 ELISA Kit

MBS722531

48-Strip-Wells

470 USD

ELISA Kit

Mouse Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 ELISA Kit

Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1

NADPH oxidase 1 isoform 1; NADPH oxidase 1; NADPH oxidase 1; mitogenic oxidase 1; NADPH oxidase homolog-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); NADPH oxidase 1; Mitogenic oxidase 1; MOX-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NOH-1

NOX1

NOX1; NOX1; MOX1; NOH1; NOH-1; GP91-2; MOX1; NOH1; NOX-1; MOX-1

NOX1_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This NOX1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NOX1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: NOX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NOX1 antibody and an NOX1-HRP conjugate. The assay sample and buffer are incubated together with NOX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NOX1 concentration since NOX1 from samples and NOX1-HRP conjugate compete for the anti-NOX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NOX1 from the sample, fewer sites are left to bind NOX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NOX1 concentration in each sample is interpolated from this standard curve.

148536873

NP_008983.2

NM_007052.4

58,972 Da

HIF-1 Signaling Pathway (695200); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); Phagosome Pathway (153910); Phagosome Pathway (153859); RAC1 Signaling Pathway (169355)

This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass; EC 1.-.-.-; Oxidoreductase; Channel, misc. Chromosomal Location of Human Ortholog: Xq22. Cellular Component: early endosome; integral to membrane; cell junction; NADPH oxidase complex. Molecular Function: protein binding; metal ion binding; superoxide-generating NADPH oxidase activity; Rac GTPase binding; NADP binding; voltage-gated proton channel activity. Biological Process: respiratory burst; intracellular pH elevation; extracellular matrix organization and biogenesis; cell migration; positive regulation of smooth muscle cell proliferation; superoxide metabolic process; positive regulation of JNK cascade; signal transduction; NADP metabolic process; regulation of systemic arterial blood pressure by renin-angiotensin; proton transport; regulation of blood pressure; positive regulation of cell proliferation; hydrogen peroxide metabolic process; angiogenesis; positive regulation of integrin biosynthetic process; inflammatory response; superoxide release

48-Strip-Wells

470 USD

96-Strip-Wells

675