ELISA/assay

Guinea pig Inducible nitric oxide synthase ELISA Kit

MBS722517

48-Strip-Wells

470 USD

ELISA Kit

Guinea pig Inducible nitric oxide synthase ELISA Kit

Inducible nitric oxide synthase

inos; Inositol-3-phosphate synthase; CG11143 gene product from transcript CG11143-RA; CG11143-PA; Inos-PA; inos; myo-inositol-1-phosphate-synthase; Myo-inositol 1-phosphate synthase; IPS; MI-1-P synthase

iNOS

Inos; Inos; bs36h12.y1; CG11143; DmelCG11143; INOS; CG11143; MIP synthase; IPS; MI-1-P synthase

INO1_DROME

Guinea Pig

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Neurobiology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This BG INOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig INOS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY INOS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-INOS antibody and an INOS-HRP conjugate. The assay sample and buffer are incubated together with INOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INOS concentration since INOSfrom samples and INOS-HRP conjugate compete for the anti-INOS antibody binding site. Since the number of sites is limited, as more sites are occupied by INOS from the sample, fewer sites are left to bind INOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INOS concentration in each sample is interpolated from this standard curve.

17137626

NP_477405.1

NM_058057.6

62,249 Da

Inositol Phosphate Metabolism Pathway (3024); Inositol Phosphate Metabolism Pathway (1006205); Inositol Phosphate Metabolism Pathway (362); Metabolic Pathways (132021); Metabolism Pathway (1006204); Synthesis Of IP2, IP, And Ins In The Cytosol Pathway (1006210)

48-Strip-Wells

470 USD

96-Strip-Wells

675