ELISA/assay

Human Cystatin D (CST5) ELISA Kit

MBS7225016

48-Strip-Wells

470 USD

ELISA Kit

Human Cystatin D (CST5) ELISA Kit

Cystatin D (CST5)

cystatin-D; Cystatin-D; cystatin-D; cystatin 5; cystatin-5; cysteine-proteinase inhibitor; cystatin D; Cystatin-5

CST5

CST5; CST5

CYTD_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of CSTD. No significant cross-reactivity or interference between CSTD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CSTD and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CSTD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CSTD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the assay: CSTD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CSTD antibody and an CSTD-HRP conjugate. The assay sample and buffer are incubated together with CSTD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSTD concentration since CSTD from samples and CSTD-HRP conjugate compete for the anti-CSTD antibody binding site. Since the number of sites is limited, as more sites are occupied by CSTD from the sample, fewer sites are left to bind CSTD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSTD concentration in each sample is interpolated from this standard curve.

19882256

NP_001891.2

NM_001900.4

16,080 Da

Endochondral Ossification Pathway (198812); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity. [provided by RefSeq, Jul 2008]

CST5: Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S cathepsin H cathepsin L cathepsin B. Belongs to the cystatin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 20p11.21. Cellular Component: extracellular region. Molecular Function: protein binding; cysteine protease inhibitor activity

48-Strip-Wells

470 USD

96-Strip-Wells

675