ELISA/assay

Human High sensitivity C Reactive Protein ELISA Kit

MBS722493

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human High sensitivity C Reactive Protein ELISA Kit

High sensitivity C Reactive Protein

CRP; C-reactive protein; C-reactive protein; pentraxin 1; C-reactive protein, pentraxin-related

Hs CRP

CRP; CRP; PTX1; PTX1

CRP_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of hs-CRP. No significant cross-reactivity or interference between hs-CRP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between hs-CRP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This hs-CRP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human hs-CRP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: hs-CRP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for hs-CRP. Standards or samples are then added to the microtiter plate wells and hs-CRP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of hs-CRP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for hs-CRP are added to each well to "sandwich" the hs-CRP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain hs-CRP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The hs-CRP concentration in each sample is interpolated from this standard curve.

17975775

AAL48218.2

10,415 Da

Classical Antibody-mediated Complement Activation Pathway (106409); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Selenium Pathway (198825)

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q23.2. Cellular Component: extracellular space; growth cone; extracellular region; filopodium. Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; cholesterol binding; virion binding; low-density lipoprotein binding; calcium ion binding. Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; wound healing; negative regulation of vasodilation; positive regulation of superoxide release; response to lead ion; response to hypoxia; acute-phase response; opsonization; inflammatory response; complement activation, classical pathway; aging

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675