ELISA/assay

Rat Heparin-binding Epidermal Growth Factor like-Growth Factor ELISA Kit

MBS722484

48-Strip-Wells

470 USD

ELISA Kit

Rat Heparin-binding Epidermal Growth Factor like-Growth Factor ELISA Kit

Heparin-binding Epidermal Growth Factor like-Growth Factor

heparin-binding EGF-like growth factor; Proheparin-binding EGF-like growth factor; proheparin-binding EGF-like growth factor; heparin-binding EGF-like growth factor

HB-EGF

HBEGF; HBEGF; HB-EGF; HEGFL; HB-EGF; HBEGF

HBEGF_CHICK

Rat

This assay has high sensitivity and excellent specificity for detection of HBEGF. No significant cross-reactivity or interference between HBEGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HBEGF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This HBEGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HBEGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: HBEGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HBEGF antibody and an HBEGF-HRP conjugate. The assay sample and buffer are incubated together with HBEGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HBEGF concentration since HBEGF from samples and HBEGF-HRP conjugate compete for the anti-HBEGF antibody binding site. Since the number of sites is limited, as more sites are occupied by HBEGF from the sample, fewer sites are left to bind HBEGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HBEGF concentration in each sample is interpolated from this standard curve.

4761593

AAD29416.1

22,541 Da

ErbB Signaling Pathway (83957); ErbB Signaling Pathway (458); GnRH Signaling Pathway (119321); GnRH Signaling Pathway (502)

48-Strip-Wells

470 USD

96-Strip-Wells

675