ELISA/assay

Human B cell lymphoma/leukemia 11A (BCL11A) ELISA Kit

MBS7224832

48-Strip-Wells

470 USD

ELISA Kit

Human B cell lymphoma/leukemia 11A (BCL11A) ELISA Kit

B cell lymphoma/leukemia 11A (BCL11A)

B-cell lymphoma/leukemia 11A isoform 1; B-cell lymphoma/leukemia 11A; B-cell lymphoma/leukemia 11A; EVI-9; BCL-11A; zinc finger protein 856; C2H2-type zinc finger protein; COUP-TF-interacting protein 1; ecotropic viral integration site 9 homolog; ecotropic viral integration site 9 protein homolog; B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2; BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1; B-cell CLL/lymphoma 11A (zinc finger protein); B-cell CLL/lymphoma 11A; COUP-TF-interacting protein 1; Ecotropic viral integration site 9 protein homolog; EVI-9; Zinc finger protein 856

BCL11A

BCL11A; BCL11A; EVI9; CTIP1; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL; CTIP1; EVI9; KIAA1809; ZNF856; BCL-11A; EVI-9

BC11A_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of BCL11A. No significant cross-reactivity or interference between BCL11A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCL11A and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This BCL11A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BCL11A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Epigenetics and Nuclear Signaling

Principle of the assay: BCL11A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BCL11A antibody and an BCL11A-HRP conjugate. The assay sample and buffer are incubated together with BCL11A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL11A concentration since BCL11A from samples and BCL11A-HRP conjugate compete for the anti-BCL11A antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL11A from the sample, fewer sites are left to bind BCL11A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL11A concentration in each sample is interpolated from this standard curve.

20336305

NP_075044.2

NM_022893.3

87,554 Da

This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Bcl-11A: Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Nuclear receptor co-regulator; C2H2-type zinc finger protein; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 2p16.1. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: protein homodimerization activity; protein heterodimerization activity; metal ion binding; transcription corepressor activity. Biological Process: protein sumoylation; transcription, DNA-dependent; B cell differentiation; regulation of dendrite development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of axon extension; negative regulation of transcription from RNA polymerase II promoter; negative regulation of collateral sprouting; positive regulation of collateral sprouting; negative regulation of protein homooligomerization; T cell differentiation

48-Strip-Wells

470 USD

96-Strip-Wells

675