ELISA/assay

Guinea pig Soluble Fms-like Tyrosine 1 ELISA Kit

MBS722460

48-Strip-Wells

470 USD

ELISA Kit

Guinea pig Soluble Fms-like Tyrosine 1 ELISA Kit

Soluble Fms-like Tyrosine 1

soluble fms-like tyrosine kinase 1; Flt1 protein; vascular endothelial growth factor receptor 1; flt1a; receptor tyrosine kinase Flt1b; soluble fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); Flt1 protein; Soluble Fms-related tyrosine kinase 1/soluble vascular endothelial growth factor receptor 1 isoform sFlt1-11a; Soluble fms-like tyrosine kinase 1

sFLT1

flt1; flt1; flt1b; sFlt1; VEGFR1; sFlt1; sflt1

A4QNT7_DANRE

Guinea Pig

This assay has high sensitivity and excellent specificity for detection of SFLT1. No significant cross-reactivity or interference between SFLT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SFLT1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This SFLT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig SFLT1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: SFLT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SFLT1 antibody and an SFLT1-HRP conjugate. The assay sample and buffer are incubated together with SFLT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SFLT1 concentration since SFLT1 from samples and SFLT1-HRP conjugate compete for the anti-SFLT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SFLT1 from the sample, fewer sites are left to bind SFLT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SFLT1 concentration in each sample is interpolated from this standard curve.

324499394

ADY39752.1

53,549 Da

Adaptive Immune System Pathway (1004110); Constitutive PI3K/AKT Signaling In Cancer Pathway (1003436); Cytokine-cytokine Receptor Interaction Pathway (119260); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (1004203); DAP12 Signaling Pathway (1004204); Disease Pathway (1003331); Downstream Signal Transduction Pathway (1003616); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1004121); Downstream Signaling Of Activated FGFR Pathway (1003377)

48-Strip-Wells

470 USD

96-Strip-Wells

675