ELISA/assay

Mouse Transforming Growth Factor Alpha ELISA Kit

MBS722456

48-Strip-Wells

470 USD

ELISA Kit

Mouse Transforming Growth Factor Alpha ELISA Kit

Transforming Growth Factor Alpha

transforming growth factor alpha, partial; Protransforming growth factor alpha; protransforming growth factor alpha; TGF-alpha; transforming growth factor, alpha; EGF-like TGF; ETGF; TGF type 1

TGFa

TGFA; TGFA; TFGA; TGF-alpha; ETGF

TGFA_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of TGF-alpha. No significant cross-reactivity or interference between TGF-alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TGF-alpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Cancer

Intended Uses: This TGF-alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TGF-alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TGF-alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGF-alpha antibody and an TGF-alpha-HRP conjugate. The assay sample and buffer are incubated together with TGF-alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGF-alpha concentration since TGF-alpha from samples and TGF-alpha-HRP conjugate compete for the anti-TGF-alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by TGF-alpha from the sample, fewer sites are left to bind TGF-alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGF-alpha concentration in each sample is interpolated from this standard curve.

339542

AAA61158.1

17,329 Da

Direct P53 Effectors Pathway (137939); ErbB Receptor Signaling Network Pathway (138016); ErbB Signaling Pathway (198844); ErbB Signaling Pathway (83049); ErbB Signaling Pathway (458); FOXM1 Transcription Factor Network Pathway (137935); Glioma Pathway (83110); Glioma Pathway (522); Non-small Cell Lung Cancer Pathway (83119); Non-small Cell Lung Cancer Pathway (531)

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Membrane protein, integral; Cell cycle regulation. Chromosomal Location of Human Ortholog: 2p13. Cellular Component: extracellular space; cell surface; perinuclear region of cytoplasm; basolateral plasma membrane; integral to plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus. Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; response to drug; cell proliferation; wound healing; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675