ELISA/assay

Mouse Granulocyte Colony Stimulating Factor Receptor ELISA Kit

MBS722450

48-Strip-Wells

470 USD

ELISA Kit

Mouse Granulocyte Colony Stimulating Factor Receptor ELISA Kit

Granulocyte Colony Stimulating Factor Receptor

granulocyte colony-stimulating factor receptor; Granulocyte colony-stimulating factor receptor; granulocyte colony-stimulating factor receptor; G-CSF-R; CD114 antigen; G-CSF receptor; colony stimulating factor 3 receptor (granulocyte); CD_antigen: CD114

GCSFR

CSF3R; CSF3R; CD114; GCSFR; GCSFR; G-CSF receptor; G-CSF-R

CSF3R_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of G-CSFR. No significant cross-reactivity or interference between G-CSFR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between G-CSFR and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This G-CSFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse G-CSFR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the Assay: G-CSFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-G-CSFR antibody and an G-CSFR-HRP conjugate. The assay sample and buffer are incubated together with G-CSFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the G-CSFR concentration since G-CSFR from samples and G-CSFR-HRP conjugate compete for the anti-G-CSFR antibody binding site. Since the number of sites is limited, as more sites are occupied by G-CSFR from the sample, fewer sites are left to bind G-CSFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The G-CSFR concentration in each sample is interpolated from this standard curve.

485364

AAA63177.1

86,609 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105)

The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq, Aug 2010]

GCSFR: the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variant isoforms have been described. Protein type: Receptor, cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p35-p34.3. Cellular Component: integral to plasma membrane; extracellular region. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; receptor activity. Biological Process: neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; signal transduction; cell adhesion. Disease: Neutrophilia, Hereditary

48-Strip-Wells

470 USD

96-Strip-Wells

675