product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat alpha-Glucosidase ELISA Kit
catalog :
MBS722447
quantity :
96 Strip Wells
price :
675 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS722447
products type :
ELISA Kit
products full name :
Rat alpha-Glucosidase ELISA Kit
products short name :
alpha-Glucosidase
products name syn :
Rat a-Glucosidase ELISA Kit
other names :
alpha-glucosidase; Lysosomal alpha-glucosidase; lysosomal alpha-glucosidase; acid maltase; aglucosidase alfa; glucosidase, alpha; acid; Acid maltase; Aglucosidase alfaCleaved into the following 2 chains:76 kDa lysosomal alpha-glucosidase; 70 kDa lysosomal alpha-glucosidase
products gene name :
A-Glu
products gene name syn :
A-Glu
other gene names :
GAA; GAA; LYAG
uniprot entry name :
LYAG_HUMAN
reactivity :
Rat
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This alpha-Glu ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat alpha-Glu. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
other info2 :
Sensitivity: The sensitivity in this assay is 1.0 ng/mL.
products description :
Principle of the Assay: alpha-Glu ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alpha-Glu antibody and an alpha-Glu-HRP conjugate. The assay sample and buffer are incubated together with alpha-Glu-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha-Glu concentration since alpha-Glu from samples and alpha-Glu-HRP conjugate compete for the anti-alpha-Glu antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha-Glu from the sample, fewer sites are left to bind alpha-Glu-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha-Glu concentration in each sample is interpolated from this standard curve.
ncbi gi num :
114159823
ncbi acc num :
ABI53718.1
ncbi mol weight :
105,324 Da
ncbi pathways :
Galactose Metabolism Pathway 82931!!Galactose Metabolism Pathway 292!!Lysosome Pathway 99052!!Lysosome Pathway 96865!!Metabolic Pathways 132956!!Notch-mediated HES/HEY Network Pathway 169347!!Starch And Sucrose Metabolism Pathway 82974!!Starch And Sucrose Metabolism Pathway 344
ncbi summary :
This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
size :
96 Strip Wells
price :
675 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

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